ProtSeq:silver stained proteins

Amanda Hall (mbah@mail.newcastle.edu.au)
Wed, 25 Aug 1999 08:30:53 +1000

What I meant to put was

Is there any special 'destaining' method for silver stained gel pieces
before in-gel digestion with trypsin.

Thanks in advance

Amanda

>I have received a silver stained gel piece to carry out internal sequencing
>on. How does this differ to Coomassie stained gel pieces. Is it just a
>matter of staining some other way (I'm not sure how) and then proceeding as
>per normal or is there a special protocol.
>
>Any help and advice is gratefully received
>
>Thanks
>
>Amanda
>

Amanda Hall (BSc Hons)
Newcastle Protein
University of Newcastle
AUSTRALIA. 2308
Ph 61 2 4921-7299