Misc Affinity Resins
Roger Pearson (roger.pearson@tag.csiro.au)
Wed, 25 Aug 1999 20:11:01 +1000
Dear ABRF members,
I have purified a recombinant GST fusion protein from
E.coli inclusion bodies (~ 50 kDa in size). The protein is presently in
solution in a sodium bicarbonate/ 8M urea buffer pH 8.3 and has been
dialysed against the same buffer, previously deionised with a mixed bead
resin. When this protein was mixed with CnBr activated Sepharose 4B no
coupling occurred. Can anyone pinpoint the problem (I am sure there were no
free amines present in the buffers) Has anyone suggestions on how to
maintain solubility of this recombinant protein and achieve coupling to the
Sepharose affinity resin.
Thankyou for your help.
Roger Pearson.
Mr Roger Pearson
Tropical Agriculture
CSIRO Long Pocket Laboratories
Indooroopilly Brisbane Qld
Australia.
Tel: 07 321-42790
Fax: 07 3214-2881