Re: octylglucoside
Deb McMillen (mcmillen@morel.uoregon.edu)
Mon, 30 Aug 1999 11:50:24 -0700 (PDT)
David,
A long time ago (1982) we did some work with membrane proteins where we
swamped out the phospholipids solubilized with octyl glucoside--to track
how much residual og we had we introduced radiolabeled og at a low level
(I believe it was C14 labeled, commercially available). Worked well,
sensitivity that you require can be adjusted by how much radiolabel you
start with proportionate to the non-radiolabeled. Not chromatography, and
makes sample handling more difficult, but analysis was simple.
Deb McMillen
Institute of Molecular Biology
University of Oregon
Eugene OR
On Fri, 27 Aug 1999, Andrews, David wrote:
> Does anyone know of a good analyical assay for octylglucoside?....preferably
> a chromatographic method that's fast and simple and sensitive...not asking
> too much, am I?...
>
>
> David W. Andrews, Ph.D.
> Director, Analytical Research & Development
> BioChem Pharma, Inc.
> 30 Bearfoot Road
> Northborough, MA 01532
>
> 508-351-1004 (voice)
> 508-351-9675 (fax)
> e-mail:DAndrews@biochempharma-usa.com
>