Do you get the frozen stock? We had problems with that a year ago, but have
had no other problems since we started to defrost the bottle to be used at
room temperature for 24 hours before storing in the fridge. Again - we
found that one Lot in particular was giving us a problem. It appears that
in some Lots, there are some components which (perhaps) come out of solution
when frozen and need the time at RT to re-dissolve.
Just a possibility.
Kind Regards
Andrew
_______________________________________________________________
AstraZeneca R&D Charnwood
Molecular Biology, Bakewell Road, Loughborough, Leics, LE11 5RH, England
Tel: +44 (0)1509 644213 Fax: +44 (0)1509 645557 Mobile: +44 (0)778
8595040
andrew.walding@charnwood.gb.astra.com
-----Original Message-----
From: mcada002@mc.duke.edu [mailto:mcada002@mc.duke.edu]
Sent: 26 August 1999 22:07
To: Recipients of ABRF List
Subject: Problem gel Lot?
Has anyone had trouble with a popular gel pre-mix DNA sequencing Lot
#HD1569?
No interest in stating the manufacturer. We only want to know what your
experience has been with the Lot if you have received it. We received it
very
soon after manufactuing so it may not have gone out to many labs.
The problem:
Sequence starting showing up only after the first 350 +/- bases had
moved
past the laser. And that was with the first gel from the pack. We isolated
the
problem to the gel mix, to our satisfaction, by runnning several gels back
and
forth using new and original plates, a different Lot #, a different 377XL in
a
different lab, etc.. When a second gel from the same Lot was run on the
original plates no sequence showed at all.
We wash our plates everyday manually with 20% Multi-Terge detergent, rinse
with
hot tap water, then rinse with 18 MegaOhm H2O.. Have not seen a build up of
any
kind since we have been cleaning this way. Maybe two years?
In order to regenerate our plates, used only twice with this gel, the
manufacturer suggested soaking in 2M NaOH for 2 hours, rinse with tap water,
then rinse with 18 MegaOhm H2O. It worked but, we can't use this procedure
everyday. Also, we're not in a postion to purchase/use an industrial dish
washer which may work since it has very hot water delivered under pressure.
The question:
Are any of you using this Lot # of pre-mix gel? Is it working for you?
How are you washing your plates?
We are not trying to make it hard on the manufacturer. We only want to know
how
or if this Lot can be used effectively.
Thanks in advance,
Millie