> I run SDSPAGE to separate two proteins.Then I want to elute one of
> them.Does anybody know simple way to extract protein from acrylamide gel?
> thank you,
> fred
>
> "horizon pas nette reste ý la buvette"
One simple way, which may or may not be appropriate depending on any
subsequent assay, is to prepare a solution of 100 mM sodium acetate, 0.1% SDS
and 10 mM dithiothrietol. Add a few hundred ul to an excised spot and
agitate overnight at 37C.
Jim Mackintosh
APAF, Sydney