We are considering the purchase of a Nitrogen sensitive HPLC detector, which
senses nitrogen via chemiluminescence. This SHOULD BE a quantitative assay,
thereby affording an easy to use alternative to AAA for peptide
quantification. The only real difficulty is giving up CH3CN mobile phases
and using alcohols as organic modifiers.
The system works well with small, organic library plates, giving similar
(error ~5%) quantification to known standards. We have no experience with
peptide samples. I might imagine Arginine could be difficult to completely
pyrolyze, Lys and amide nitrogens should be no problem.
Does anyone have experience with this technique, especially with a
comparison to UV and AAA quantification?
Thanks in advance for any insight. This could remove a huge bottleneck in
our research.
David H. Singleton
Scientist
Pfizer Central Research
PO Box 8118-101
Eastern Point Road
Groton, CT 06340
-----Original Message-----
From: Breslav, Michael [PRI] [mailto:MBreslav@prius.jnj.com]
Sent: Wednesday, September 01, 1999 4:43 PM
To: Recipients of ABRF List
Subject: Nitrogen analysis.
Greetings,
I need to analyse <100 ppm of nitrogen in water. Traditionally, I believe,
this has been done by a Kjeldahl method.
I am not sure if there is anything better (like nitrogen sensitive HPLC
detector?) now for this analysis.
I am looking for this analysis as a service and would be grateful for the
directions to the service lab on an East coast.
Thanks,
Michael Breslav
R.W.Johnson PRI
Spring House, PA