Sheryl- My protocol for GC-rich templates is attached as an enclosure. This
method succeeded in sequencing Lunatic out to about 650 bases. It uses BD
chemistry- I didn't have much luck using the dGTP kit with Lunatic. Good luck.
Regards,
Allison Pinder
DNA Sequencing Core Facility
Carnegie Institution of Washington
115 W. University Parkway
Baltimore, MD 21210
(410) 554-1207
pinder@mail1.ciwemb.edu
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