I tried Sypro orange and did not realy get a good sensitivity. Can you give
me a protocol? What is rubypro?
The post-gel labelling by Ruedi Aebersolds group is that the poster using
deuterated acetamide? I think the big differences in the number of cysteins
present in proteins are quite a limitation of this method.
Best regards,
Juri Rappsilber
Protein Interaction Laboratory
University of Southern Denmark
>I'll modify my answer a little and say, the methods shown by Ruedi
Aebersolds group are the way to go. We have a more generalised method which
can quantitate any gel spot (relative to an internal standard) without the
need for either isotopic labelling during growth, but only needing 2D gel
spots and post-gel labelling. As to rubypro, it works, the sypro did not. As
to why, hard to tell without the structures of the stains. This is a merely
rule of thumb observation. Sypro orange was very sensitive to the type of
protein being stained, even more so than silver.
PS A UV lightbox only costs 100$ US; a pair of mountaineers glasses about
the same again. Even if the stain does not suite you at least you can go on
holiday with an easy conscience.
ciao
Peter<
_|__'
\|
___________________________ _____________________________
Juri Rappsilber
Protein Interaction Laboratory
University of Southern Denmark
Campusvej 55
DK-5230 Odense M, Denmark
Phone: +45 6550 2435 (MS Lab)
+45 6550 2474 (MS office)
Fax: +45 6593 3018 (Secretary)
E-mail: rappsilber@cebi.sdu.dk