Has someone of you some experience in using TCEP
(tris-carboxy-ethylphosphine) for 2D-electrophoresis ?
What concentration do you use in the rehydrationbuffer/equilibrationbuffer.
Doe you have some protocol ?
Are there some special things to account for ?
I'm trying to get a good 2D-separation of keratins; that's why I want to
try TCEP instead of DTT. Is someone familiar with 2D-electrophoresis of
keratins. What do you do to get a good result ?
Thanks in advance for answering !
Greetings
Annick
Annick Dani?ls,
Innogenetics N.V.
Industriepark Zwijnaarde 7, box 4
B-9052 Gent
Belgium
Phone : 0032-9-241.07.77. or 78
e-mail : annicdan@innogenetics.be