Re: PVDF and Nylon transfer membranes

Ricardo Bastos Cunha (rbcunha@unb.br)
Wed, 08 Sep 1999 08:23:06 -0300

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Bryan Dunbar wrote:
> what's the general feeling about the variability between different
> suppliers of these membranes?. The obvious sources that come to mind are
> PE/Bio, Millipore, Bio-Rad and Schliecher and Schuell. I've never used nylon
> myself. I would be glad to hear of any good/bad experiences.
> Regards from Scotland.
> Bryan

If you want to put your membrane into a protein sequencer do not use
nylon, because it will be melted by TFA.

Best regards.

-------------------------------------------------------
Prof. Ricardo B. Cunha
Brazilian Center for Protein Research and Services - CBSP
Laboratory of Protein Chemistry and Biochemistry
Departament of Cell Biology
University of Brasilia
Brasilia - DF Brazil
CEP 70910-900

Tel: 55-21-61-307-2142
Fax: 55-21-61-272-4548
mailto: rbcunha@unb.br
http://www.lbqp.unb.br/ricardo
http://www.unb.br/cbsp
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