Electrophoresis 1998 May;19(5):845-51
Improved protein solubility in two-dimensional electrophoresis using tributyl
phosphine as reducing
agent.
Herbert BR, Molloy MP, Gooley AA, Walsh BJ, Bryson WG, Williams KL
they use tributylphosphine which worked very nicely. it is not easy to handle
though as it decomposes pretty rapidly... separation patterns improveover the
DTT approach though.
good luck, dorian