I believe the article you are referring to is:
Proc. Natl. Acad. Sci. USA 83, 6233-6237 1986
Best regards,
Ron
-----------------------------------------------
Ronald C. Hendrickson, Ph. D.
Corixa Corporation
1124 Columbia Street, Suite 200
Seattle, WA 98104 USA
206.754.5811 (phone)
206.754.5715 (fax)
-----Original Message-----
From: David Arnott [mailto:arnott@gene.COM]
Sent: Wednesday, September 08, 1999 9:57 AM
To: Recipients of ABRF List
Cc: Recipients of ABRF List
Subject: Re: esterification of peptides
Hello Jeff. I don't have a literature reference for the procedure, but here
is
a protocol which I learned in grad. school and has been very reliable.
1. Speed-vac the sample to dryness.
2. Prepare 2N methanolic or ethanolic HCl by drowpise addition of 60 uL
acetyl choride to 300 uL anhydrous methanol or ethanol in a glass test tube.
Cover with parafilm and let stand 10 min. Warning: exothermic reaction -
use
safety glasses, work in hood with the tube opening pointed away.
3. Add 15 uL of the reagent to each sample and let stand at room temp. for
90
min (methyl esters) or 120 min. (ethyl esters).
4. Dry the sample on a speed-vac.
5. Redissolve the modified peptides.
Esterification of each carboxylic acid group increases the mass of a peptide
by
14 Da for methyl esters or 28 Da for ethyl esters.
Good luck
David
JWU388880@cs.com wrote:
> Hi,
> Does anybody know the protocol (or recipe) for the direct esterification
of
> tryptic digests (convert -COOH to -COOCH3). The chemistry has been used
to
> facilitate mass spec identification of peptides. Thanks a lot.
> Jeff.