gsarath@unlnotes.unl.edu wrote:
> Dear All: I recently synthesized a 17 mer (1R, 3K, 3Y, 1D, 2E, 1S, 1T, 1N, 1P,
> 2V, 1A), acetylated at the N-terminus using acetylimidazole, C-terminus was
> amidated. Synthesis looked normal, as did the yields of the crude peptide.
> Analytical HPLC showed two close peaks, I took the major one to be N-blocked (it
> was). However, the mass is +40 of the expected, any ideas?? Thanks in advance
> for the wisdom, Gautam Sarath
-- Matteo Villain E-mail : villain@uab.edu Research Associate Phone : 205 934 3032 University of Alabama Fax : 205 934 1446 Birmingham 35294, USA