Y G R K K R R Q R R R G K P P A P P M R N T S T M
Before labeling, we did a partial cleavage to make sure we made the intact
peptide and it looks great.
We tried using FITC followed by washing and cleaving. We end up with ONLY
the peptide - Y. I figure I must be going through an edman type cleavage
during cleavage and deprotection even though we keep it pretty cool. (I
know some are saying DAH!)
We then used Fluorescein-NHS. Most of our product is unlabeled (90%) and
then there is a product that is that is -17 amu.
What is your favorite protocol for labeling with Fluorescein?