pI determination of basic peptides

Corrado Guarnaccia (guarna@icgeb.trieste.it)
Wed, 15 Sep 1999 16:42:37 +0200 (MET DST)

Hi everybody,

we need to compare the pI of two very basic peptides (theoretical pI over
12); does anybody of you have experience or know about the best method to
do it? It seems that the classical isoelectrofocusing and chromatofocusing
techniques stop at pH 10-11.

Thanks a lot for any help

Corrado Guarnaccia
ICGEB - Padriciano 99
Trieste - ITALY
E-Mail: guarna@icgeb.trieste.it