dbEST

Matteo Villain (villain@uab.edu)
Wed, 15 Sep 1999 09:26:59 -0500

I have a question regarding the dbEST.( Expressed Sequence Tags).
The DNA sequence present in this database are stretch of mRNA. There is
any way to determine the reading frame (the logical way I thought was
the reading frame should be the one without stop codons, but since the
stop are only 3 out of 64, I think the probability to get three complete
translations are too high). And the other question is, are the sequence
always deposited as sense DNA, meaning when you create this DNA, do you
know which is the sense strand.
Thank you for any answer.
Matteo

--
Matteo Villain                   E-mail : villain@uab.edu
Research Associate               Phone  : 205 934 3032
University of Alabama            Fax    : 205 934 1446
Birmingham
35294, USA