Corrado-
A representative of Kendrick Laboratories once told me that they can examine relative pIs of very basic proteins by stopping the isoelectric focusing before the proteins reach their pIs (i.e. while they're still moving). One can monitor the progress of the electrophoresis by including cytochrome C, which is a visible high-pI protein, in one of the lanes.
I've never tried this, but would be very interested in knowing how well the technique works.
Vernon
Vernon A. Shoup
Regeneron Pharmaceuticals
Rensselear, New York 12144
USA
vernon.shoup@regpha.com
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Date: 09/15/1999 1:18 PM
To: VERNON SHOUP
From: Corrado Guarnaccia
Hi everybody,
we need to compare the pI of two very basic peptides (theoretical pI over
12); does anybody of you have experience or know about the best method to
do it? It seems that the classical isoelectrofocusing and chromatofocusing
techniques stop at pH 10-11.
Thanks a lot for any help
Corrado Guarnaccia
ICGEB - Padriciano 99
Trieste - ITALY
E-Mail: guarna@icgeb.trieste.it