Lys problem
Pawel Mak (makp@mol.uj.edu.pl)
Tue, 21 Sep 1999 15:19:01 +0200
Dear Colleagues,
I have a question for protein sequencing experts: what may be cause of
disappearing of lysine in sequenced samples? I use Applied Biosystems 491
system and all is working perfectly: the instrument sequences samples very
well, the level of lysine in standards is about 1.5 greater than level of
leucine but mysteriously the potential lysine residue in sequenced proteins
gives a blank run (in fact I can see lysine but it is very, very small peak
and additionally before peC as well as after V appear small, unidentified
peaks). Of course I know that the oxidants in A or in R4 can partially
oxidise lysine but PARTIALLY... I also do not know if the very high level
of DPU I observe on all chromatograms may be related to lysine problems ?
Thank you very much for any suggestions !!!!
Pawel Mak
Dr. Pawel Mak
Jagiellonian University
Institute of Molecular Biology
Mickiewicza 3
31-120 Krakow
POLAND
e-mail: makp@mol.uj.edu.pl
WWW: http://www.mol.uj.edu.pl/biocentrum
fax (+48)(012)6336907