RE: Different PI proteins

Chin, David T. (ChinD@missouri.edu)
Tue, 21 Sep 1999 09:36:47 -0500

Bob,
IEF and CE come to mind if you have protein standards. If they are
identical except on the order of the residues, the pI will likely be close
to each other, thus a good CE protocol may do you better than IEF.
Alternatively you can have them sequenced.

David

David T. Chin
Director, Protein Core Facility
Protein Chemistry and Expression
2-17 Agriculture Building
Univ. of Missouri - Columbia
Columbia, MO 65211-7170

SHIPPING AND ACTUAL LOCATION:
2-17 Agriculture Building (office)
2-31 Agriculture Building (Lab)

e-Mail: chind@missouri.edu
web: http://www.biotech.missouri.edu/pc
[mailto:chind@missouri.edu]
Phone: 573-882-2027
Fax: 573-882-7105

-----Original Message-----
From: Bob Lee [mailto:boblee@uic.edu]
Sent: Monday, September 20, 1999 4:53 PM
To: Recipients of ABRF List
Subject: Different PI proteins

Hi, everyone. Need suggestions on ways to identify two identical
(composition) proteins with differnt PI.

Bob