A very interesting paper has recently been published concerning phosphopeptide isolation using gallium IMAC. Posewitz and Tempst, Anal. Chem. 71:2883-2892 (1999),
Caveat, be cautious when using gallium chloride...fume hood and glasses, gloves and lab coat.
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Gordon Alton, PhD
Gene and Protein Discovery Group
Signal Pharmaceuticals Inc.
5555 Oberlin Drive
San Diego, CA 92121
Email: galton@signalpharm.com
Phone: 619-558-7500 x8252
Fax: 619-623-0870
WWW: http://www.electriciti.com/signal/
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-----Original Message-----
From: Len Packman [SMTP:lcp2@mole.bio.cam.ac.uk]
Sent: Friday, September 24, 1999 9:27 AM
To: Recipients of ABRF List
Subject: IMAC
Dear ABRFers
Does anyone have any useful protocols for microisolation of phosphopeptides
using micro IMAC columns, especially from gel-digested material? Any
suggestions about sourcing material, making columns, recipes and
sensitivity issues gladly received.
Len
*********************************************************************
Dr Len C. Packman
Assistant Director of Research
Protein and Nucleic Acid Chemistry Facility
Department of Biochemistry
University of Cambridge
80 Tennis Court Road
Old Addenbrookes Site
Cambridge, CB2 1GA, UK
Tel: +44 (1223) 333639
FAX: +44 (1223) 766002
e-mail: lcp2@mole.bio.cam.ac.uk
Visit my WWW page at http://www.bio.cam.ac.uk/proj/adr/PNAC/pnac.html