I have recently received the following query from an investigator:
"I am interested in sequencing proteins that have been
nitrated at tyrosine moieties. Can your detection process
differentiate between tyrosine and nitrotyrosine?"
I do not find a retention time for nitrotyrosine in the "Identification
of Modified PTH-Amino Acids in Protein Sequence Analysis" that all of us
rely on. Before I tell the PI "NO", I thought I'd send the query to
you-all and see if anyone knows of a way to do it, or alternately the
reason that it is not feasible.
Thank you in advance for your help!
-- Rebecca P. Ettling
Biotechnology Resource Laboratory
Protein Sequencing and Peptide Synthesis Facility
Medical University of South Carolina
173 Ashley Avenue, Room 733D BSB
Charleston, SC 29403
Tel.: (843) 792-1271
Fax: (843) 792-1264
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I have recently received the following query from an investigator:
"I am interested in sequencing proteins that have been nitrated at tyrosine moieties. Can your detection process differentiate between tyrosine and nitrotyrosine?"I do not find a retention time for nitrotyrosine in the "Identification of Modified PTH-Amino Acids in Protein Sequence Analysis" that all of us rely on. Before I tell the PI "NO", I thought I'd send the query to you-all and see if anyone knows of a way to do it, or alternately the reason that it is not feasible.
Thank you in advance for your help!
-- Rebecca P. Ettling
Biotechnology Resource Laboratory
Protein Sequencing and Peptide Synthesis Facility
Medical University of South Carolina
173 Ashley Avenue, Room 733D BSB
Charleston, SC 29403
Tel.: (843) 792-1271
Fax: (843) 792-1264
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