Goodwin, DC, Gunther, MR, Hsi, LC, Crews, BC, Eling, TE, Mason, RP,
Marnett, LJ:
Nitric oxide trapping of tyrosyl radicals generated during prostaglandin endoperoxide
synthase turnover. J Biol. Chem. 273:8903-8909, 1998.
We worked on this technique using the Beckman/Porton LF3400 with
diodearray detection.
Contact me if you have some questions on this.
Eric
On Mon, 27 Sep 1999 10:25:07 -0400 Rebecca Ettling <ettlingr@musc.edu>
wrote:
> I have recently received the following query from an investigator:
>
> "I am interested in sequencing proteins that have been
> nitrated at tyrosine moieties. Can your detection process
> differentiate between tyrosine and nitrotyrosine?"
>
> I do not find a retention time for nitrotyrosine in the "Identification
> of Modified PTH-Amino Acids in Protein Sequence Analysis" that all of us
> rely on. Before I tell the PI "NO", I thought I'd send the query to
> you-all and see if anyone knows of a way to do it, or alternately the
> reason that it is not feasible.
>
> Thank you in advance for your help!
> -- Rebecca P. Ettling
> Biotechnology Resource Laboratory
> Protein Sequencing and Peptide Synthesis Facility
> Medical University of South Carolina
> 173 Ashley Avenue, Room 733D BSB
> Charleston, SC 29403
> Tel.: (843) 792-1271
> Fax: (843) 792-1264
>
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Howard, Eric F
Vanderbilt University
Email: eric.f.howard@Vanderbilt.Edu
Protein Sequencing and Amino Acid Analysis Laboratory
615-322-6355