Genotyping - info request

Robert Lyons (boblyons@umich.edu)
Wed, 29 Sep 1999 09:45:46 -0400

I would like to tap into your genotyping expertise, if I could.
The University of Michigan has not yet offered a genotyping service
(much to the consternation of our many geneticists). For various
reasons, we may begin such a service in the not-too-distant future.
Because I operate automated sequencers, it would be easy for me
to expand operations to include GeneScan work. However, I am badly
uninformed and inexperienced with genotyping, and need some info
to help me decide how to proceed:

- Can someone who routinely does GeneScan runs on ABI sequencers
please give me a rough estimate of typical cost per marker?
(the real operational costs, assuming we'd buy commercially-
available primer sets).

- SNP-based genotyping seems to hold great promise for the
future. Would I be foolish to start up gel-based genotyping
instead of, say, array hybridization against SNP oligos? or
one of the other new SNP-based technologies?

Thanks a million for any clarification or discussion.

Bob Lyons
University of Michigan

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Robert Lyons, Ph.D.
Director, DNA Sequencing Core
University of Michigan
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