Re: Prot Seq: Using Novex NuPage gels and pvdf products
Bryan Dunbar (bch143@abdn.ac.uk)
Wed, 29 Sep 1999 16:36:30 +0100
>From: Bryan Dunbar <bch143@abdn.ac.uk>
>Subject: Re: Prot Seq: Using Novex NuPage gels and pvdf products
>
>Tom,
> I have been using the NuPage gradient gels for about a year now, I
guess. The resolution is superb, I see PAM-CYS, I don't see any strange PTH
peaks . I don't use their PVDF, I use ABI's Problott. I get good data
using NuPage transfer buffer and Amido Black stain. They do take longer to
transfer - with my home-made 0.75mm gels, everything had transferred at
250mA for 1 and a half hours. The NuPage 1.0mm takes 2 and a half hours. I
have two pulsed-liquid 477A's, but my recollection is that gas-phase worked
ok (one 477A was modified at one time to deliver g-p R3). I changed it back,
but not because of the NuPage stuff - just a personal preference.
>
>At 15:38 24/09/99 -0400, you wrote:
>>Hello. I am receiving blots using Novex NuPage gels and their
>>pvdf product. Has anyone seen good results using these products
>>on gas-phase protein sequencers? Any caveats or shortcomings?
>>Thanks for any comments.
>>Regards, Tom
>>
>>*****************************************
>>Thomas Miller
>>DuPont Company
>>Protein Sequencing Lab / Oligo synthesis
>>email: Thomas.J.Miller@usa.dupont.com
>>phone: 302-695-1745
>>
>>Opinions are my own and not of my employer
>>
>>
>>
>>
>
Bryan Dunbar
Protein Facility,
Room WT27,
Department of Molecular & Cell Biology,
University of Aberdeen,
Polwarth Building,
Foresterhill,
Aberdeen,
SCOTLAND.
U.K.
AB25 2ZD.
TEL: 01224-273103
FAX: 01224-273104
e-mail; b.dunbar@abdn.ac.uk