Re: BAC DNA sequencing

Bernd Weisshaar (weisshaa@mpiz-koeln.mpg.de)
Fri, 1 Oct 1999 15:38:51 +0200

We had usually good results with DNA prepared with the Qiagen kit
(they have a BAC protocol). However, also in this case there was some
"user variation" in terms of quality ..... the results from
differerent users using the same kit were not always good. Also,
http://bacpac.med.buffalo.edu/ has protocols.

Regards, Bernd

>Does anyone out there have a good protocol to use for cleaning up BAC DNA
>for automated sequencing. I am having problems sequencing most people's
>BAC - even using the longer cycling times.
>
>Thanks in advance
>
>Biopolymer Lab
>UMAB, Howard Hall Rm. 323
>Mailing Address:
>Department of Microbiology and Immunology
>13-009 Bressler Bldg.
>655 W. Baltimore Street
>Baltimore, MD 21201
>(410) 706-3339 - phone
>(410) 706-0287 - fax

_____________________________________________________________________

PD Dr. Bernd Weisshaar
MPI fuer Zuechtungsforschung
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50829 Koeln
Germany

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