On Waters Pico Tag system, ( also using PTC chemistry ), Alanine and Methionine sulfoxide elute between citrulline and arginine.
Hope this will help.
Andrzej
>>> Sandy Kielland <kielland@UVic.CA> 10/01/99 03:50PM >>>
Hello all;
I have a modified amino acid eluting on my PTC profile between citrulline
and arginine. I'm using an ABI420. The retention time of citrulline is 8.12
min, the mystery amino acid is at 8.33 and arg is 8.58 min. The sample is a
cyclic peptide which we have been unable to cleave for sequencing. Has
anyone seen a mod-AA elute in this area before?
Thanks in advance for any input;
Sandy Kielland
Victoria Protein Microchemistry Centre
Department of Biochemistry and Microbiology
University of Victoria
Victoria B.C. CANADA
phone: (250) 7218884
FAX (250) 7218855
Email Kielland@UVic.ca
Webpage http://proteincentre.dhs.org
Andrzej Pastuszyn Ph.D.
Department of Biochemistry and Molecular Biology
University of New Mexico
Albuquerque, NM 87131-5221
Tel: 505-272-4305
Fax: 505-272-6587
e-mail:apastuszyn@salud.unm.edu