Lectins

Andreas Franz (afranz_bus@yahoo.com)
Tue, 12 Oct 1999 09:04:15 -0700 (PDT)

Hi,

answers that I received concerning my question from
last week were only forwarded to my personal account.
Here is the information in my own words (since I do
not have the original messages any more):

Literature:

SIGMA catalog + references cited therein

"Advances in lectin research", Vol 1+2, VEB Verlag
Volk und Gesundheit, Berlin 1988

"Carbohydrate Analysis: A Practical Approach", M. F.
Chaplin, J. F. Kennedy ed.

In general, the most common procedure is to elute
carbohydrates from lectins by means of a competitive
ligand such as mono- or disaccharides at high
concentration. Different lectins require differnt
haptens. ConA e.g. requires 0.3M
methyl-alpha-D-glucoside whereas WGA requires 0.1-1.0M
N-acetyl-glucosamine.

Hope this helps.

Thanks again

Regards

Andreas Franz

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