Database searching help request

Suzanne Perry-Riehm (perry@interchange.ubc.ca)
Thu, 14 Oct 1999 11:59:11 -0700 (PDT)

I have a client who requested that I search specifically databases looking
for beta- mannosidases. He cited known sequences of beta mannosidases from
Human, Capra hircus, Bos taurus and Aspergillus. I performed a digestion
and generated 6-8 peptide
sequences to which he requested I use in the search. The origin of the
digested
protein is Trichoderma reesei EC 3.2.1.25.

Does anybody out there know if this is possible or have suggestions as to
how to at least attempt a similar search for this client? I've performed a
general NR database search but I suppose he would like a more specific
and limited search.

Thanks in advance for any and all suggestions!
Suzanne Perry-Riehm

Suzanne C. Perry, Research Associate
NAPS Protein Sequencing and Peptide Mapping Laboratory
Biotechnology Department, University of British Columbia
CANADA
phone:(604)822-9688( direct) or (604)822-9662 (main) Fax:(604)822-0676
email:perry@interchange.ubc.ca
Visit our web page at : www.biotech.ubc.ca/naps/napshome.htm