It is certainly possible to silver stain IEF gels, since there are
commercial kits available.
As an alternative, Molecular Probes makes a product "SYPRO Ruby IEF",
which apparently has sensitivity and a dynamic range that exceeds
silver staining, plus is simpler to use. All you need is a UV box to
visual the stained gel. (info at www.probes.com/syprodyes).
(note that I haven't yet tested this dye myself , although I have
been very happy with other Molecular Probes products)
Hope this is useful.
>I would like to know if it is possible to silver stain ampholine IEF
>gels, not IPG. A good reference would be great.
>
>Thanks in advance,
>
>Jaime Paba
>Universidade de Brasilia
>Brazil
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