RE: Tyrosine sulfation

Klein, Mike L. (mklein@amgen.com)
Fri, 15 Oct 1999 16:13:03 -0700

Kristine-
Traditional denaturation/alkylation (I'm assuming you're talking about pH
7-9) will not affect the sulfation state of your sulfotyrosines. It's
strong acid you have to worry about; even normal exposure to 0.1% TFA in a
reversed-phase column won't be much trouble, either.
-Mike
Michael L. Klein, Ph.D.
Research Scientist
Analytical Research & Development
Amgen, Inc.
One Amgen Center Drive
M/S 25-2-A
Thousand Oaks, CA 91320
(805) 447-2773
FAX (805) 447-8690
mklein@amgen.com

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From: Kristine Swiderek [SMTP:swiderek@zgi.com]
Sent: Friday, October 15, 1999 2:18 PM
To: Recipients of ABRF List
Subject: Tyrosine sulfation

Dear all,

How would a classical reduction and alkylation step (denaturing
conditions,
DTT, iodoacetic acid) prior to protein digestion affect a sulfated
tyrosine?

Any input is appreciated, Kristine

Kristine Swiderek
Assoc. Research Director
Department of Protein Biochemistry
ZymoGenetics, Inc.
1201 Eastlake Ave. E
Seattle, WA 98102
Phone: (206)515-4901
Fax: (206)442-6608
e-mail: swiderek@zgi.com