I am interested in using gel mobility shift electrophoresis to study
nucleic acid - drug interactions. Does anyone know the answers to any of
the following questions that I have?
In general ...
1) how long does it usually take to run one experiment?
2) how quantifyable are the results?
3) how automatable is the technique?
4) what are the limitations of the technique (what types of experiments
tend to fail)?
5) how reproducible are the results?
Thanks in advance for any replies to my questions. I think this
information will be useful to many people to have as a general review
and learning document, and I will post any "answers" sent to me in the
hopes that ABRF will add the final review document to its archives.
Dr. Mark McGovern
Vice President of Technology
SensorChem International Corp
Toronto, Canada