I and others are interested in getting HRMS on compounds like carbohydrates
both protected monosaccharides and larger structures like oligosaccharides,
glycopeptide derivatives and so on for publication purposes. We have an old
FAB instrument, CI instrument (although we dont have a low temp probe) and
have a new electrospray which appears to be very good but not suitable for
High Res or at least its very difficult according to our operator. There
is also the possibility that our institute will be purchasing an instrument
for analysing proteins and glycoproteins/glycopeptides and so on in the
near future.
At a previous institute where I worked the operator was able to do HRMS on
most compounds I gave him using FAB and he he used a special low
temperature probe for CI experiments on most of the compounds (e.g
benzylated and acetylated carbohydrates) I gave him and FAB on compounds
that were not suitable for CI which were unprotected polar carbohydrates.
I have written to him but would also like to hear any other comments.
We would be grateful here if anyone has any suggestions about doing HR
experiments in general with any of the above techniques or perhaps there
are newer tecniques more suitable?
Is there is any new equipment available where these experiments might be
easier to do?
Has anybody got any information on low temperature CI probes that we could
purchase which might help us.
Has any one got suggestions about purchasing new equipment for proteins and
larger carbohydrates and glycopeptides.
Has anyone any comments at all that would help us be better informed before
we go about trying to fix the problems or before purchasing new equipment.
With best regards and thanks for your help
Paul
Dr. Paul V. Murphy
Department of Chemistry,
University College Dublin,
Belfield,
Dublin 4
Tel. +353-(0)-1-7062504
Fax. +353-(0)-1-7062127
email: Paul.V.Murphy@ucd.ie
http://www.ucd.ie/~chemist/PMurphy.html