RE: BigDye Cleanup

Cariola, Michael L. (CARIOLAM@afip.osd.mil)
Thu, 21 Oct 1999 13:12:12 -0400

I recommend Edge Biosystems AGTC gel filtration cartridges (cat# 42453).
You can contact them at 1-800-326-2685 or www.edgebio.com
<http://www.edgebio.com> . Cost is around $1/column.

Michael Cariola
Quality Control Supervisor
Armed Forces DNA Identification Laboratory
1413 Research Blvd., Bldg. 101
Rockville, MD 20850
Office: (301) 319-0253 FAX: (301) 295-5932
e-mail: cariolam@afip.osd.mil <mailto:cariolam@afip.osd.mil>

-----Original Message-----
From: Phyllis Spatrick [SMTP:pspatric@sunspot.ummed.edu]
Sent: Thursday, October 21, 1999 10:27 AM
To: Recipients of ABRF List
Subject: BigDye Cleanup

Hello Everyone
We recently switched to using big dyes from the TaqFS. We are
finding
that our sample cleanup method of G50 beads packed into a spin
column is
not removing enough of the dye now. We tried a Princeton separations

column which was much better, but that seemed akward and it is
expensive.
What do other labs use? Any Suggestions?
Phyllis

Phyllis Spatrick
University of Massachusetts - Worcester Campus
Phyllis.Spatrick@ummed.edu