MS:Best stain for sub-pmole digests

lisa bibbs (bibbs@scripps.edu)
Thu, 21 Oct 1999 12:44:20 -0700

What is the best stain for sub-pmole amounts of protein to be in-gel
digested followed by Mass spec identification (MALDI followed by
LC-MS)? I have heard that Silver staining can be very bad and
produce no MALDI results. We haven't gone to sub-pmole yet so we
have just been using Coomassie and such.

Thanks for your help.

Lisa
Lisa Bibbs, Ph.D.
10550 N. Torrey Pines Rd.
SP3
La Jolla, CA 92039

http://corefac.scripps.edu