What about some of the fluorescent dye markers such as SYPRO Orange? I
haven't used them myself but lots of my colleagues swear by them!
Cheers,
Roger
At 12:44 PM 21-10-99 -0700, you wrote:
>What is the best stain for sub-pmole amounts of protein to be in-gel
>digested followed by Mass spec identification (MALDI followed by
>LC-MS)? I have heard that Silver staining can be very bad and
>produce no MALDI results. We haven't gone to sub-pmole yet so we
>have just been using Coomassie and such.
>
>Thanks for your help.
>
>Lisa
>Lisa Bibbs, Ph.D.
>10550 N. Torrey Pines Rd.
>SP3
>La Jolla, CA 92039
>
>http://corefac.scripps.edu
------------------------------------------------------------
Roger Murphy, Ph.D.
Biological Production Facility
Ludwig Institute for Cancer Research
Austin & Repatriation Medical Centre
Studley Road,
Heidelberg, Vic. 3084
Australia.
Tel 61-3-94965463
Fax 61-3-94965436
Email Roger.Murphy@Ludwig.edu.au