I gotta throw this query from a researcher on the table. I know there is some missing info like amounts but..
Anyone with a suggestion or reference ?
-Paul
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Is there a chemical way to dephosphorylated pser? We generated an antibody and want to do competitions, but only made the phosphorylated form...and are too cheap to make another one. It needs to be complete (we've tried the enzymatic route with less than stellar success)-any residual might be enough to block the signal.
Thanks
John
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____________________________________ Paul Morrison JFB216 paul_morrison@dfci.harvard.edu Molecular Biology Core Facilities Dana-Farber Cancer Institute http://mbcf.dfci.harvard.edu 44 Binney Street 617-632-3082 Boston, MA 02115 fax 632-4814 ____________________________________