Hi Tim,
Didn't know you were on this forum! Never have solved this
problem; if you don't get a good answer one thing to do is take a
cheap, small disulfide containing peptide such as vasopressin,
oxytocin, or our "AVP-like" factor from Locusta migratoria, reduce it
and alkylate with your reagent of choice, and then sequence it. You
will get modified Cys residues on Cycles 1 and 6. Since the amount
of sample is basically unlimited compared with the types of proteins
you will be sequencing, you really only need to run the first cycle
usually. Can go out to six to double check.
Let me know if you want some of our peptide from Locusta.
Seqeunce is CLITNCPRG-NH2
David
David A. Schooley
Dept. of Biochemistry/330
Univ. of Nevada
Reno, NV 89557
schooley@unr.edu
tel: (775) 784-4136; fax (775) 784-1419
NOTE NEW AREA CODE: Mandatory after 5/15/99
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Content-Type: text/enriched; charset="us-ascii"
<fontfamily><param>Geneva</param>Hi Tim,
Didn't know you were on this forum! Never have solved this problem;
if you don't get a good answer one thing to do is take a cheap, small
disulfide containing peptide such as vasopressin, oxytocin, or our
"AVP-like" factor from Locusta migratoria, reduce it and alkylate with
your reagent of choice, and then sequence it. You will get modified
Cys residues on Cycles 1 and 6. Since the amount of sample is
basically unlimited compared with the types of proteins you will be
sequencing, you really only need to run the first cycle usually. Can go
out to six to double check.
Let me know if you want some of our peptide from Locusta. Seqeunce is
CLITNCPRG-NH2
David
</fontfamily>
David A. Schooley
Dept. of Biochemistry/330
Univ. of Nevada
Reno, NV 89557
schooley@unr.edu
tel: (775) 784-4136; fax (775) 784-1419
NOTE NEW AREA CODE: Mandatory after 5/15/99
--============_-1271414496==_ma============--