We have had mixed results with silver staining and mass spec, but in
general if you employ some type of sample clean-up e.g. Zip Tips, you can
obtain reasonable spectra. There is also a new fluorescent stain on the
market - Sypro Ruby. My experience shows it to be as sensitive as silver
staining and much better than Sypro orange. I have tried mass spec from
sypro without sample clean-up and the spectra is not great, but again with
a zip-tip, I got good results. It is manufactured by Molecular Probes and
sold directly by them or Genomic Solutions and Biorad.
Cheers,
Nikki.
At 12:44 21/10/99 -0700, you wrote:
>What is the best stain for sub-pmole amounts of protein to be in-gel
>digested followed by Mass spec identification (MALDI followed by
>LC-MS)? I have heard that Silver staining can be very bad and produce no
>MALDI results. We haven't gone to sub-pmole yet so we have just been
>using Coomassie and such.
>
>Thanks for your help.
>
>Lisa
>Lisa Bibbs, Ph.D.
>10550 N. Torrey Pines Rd.
>SP3
>La Jolla, CA 92039
>
>http://corefac.scripps.edu
Nicole Verrills
APAF
Building F7B Room 431
Macquarie University
Sydney, Australia 2109
Ph: 61-2-9850-6219
FAX: 61-2-9850-6200
Email: nverrill@proteome.org.au
http://www.proteome.org.au