RE: ProtSyn kinda: dephosphorylation of PSer

HOWARD, SUSAN C [PHR/1005] (susan.c.howard@stl.monsanto.com)
Mon, 25 Oct 1999 08:21:36 -0500

Paul,
You guys are being silly--pennywise and pound foolish so to speak. Just make
the nonphosphorylated control. If your time has any monetary value at all,
by the time it's all said and done, you will come out ahead, especially
since you are concerned that trace amounts of phosphorylated peptide could
still cause problems.
Susan C. Howard
Searle

-----Original Message-----
From: Paul Morrison [mailto:paul_morrison@dfci.harvard.edu]
Sent: Friday, October 22, 1999 1:49 PM
To: Recipients of ABRF List
Subject: ProtSyn kinda: dephosphorylation of PSer

Dear ABRFers,

I gotta throw this query from a researcher on the table. I know there is
some missing info like amounts but..

Anyone with a suggestion or reference ?

-Paul

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Is there a chemical way to dephosphorylated pser? We generated an antibody
and want to do competitions, but only made the phosphorylated form...and are
too cheap to make another one. It needs to be complete (we've tried the
enzymatic route with less than stellar success)-any residual might be enough
to block the signal.
Thanks
John
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Paul Morrison   JFB216               paul_morrison@dfci.harvard.edu
Molecular Biology Core Facilities    
Dana-Farber Cancer Institute         http://mbcf.dfci.harvard.edu
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