safe use of acrylamide/polyacrylamide

A.Krupa (A.Krupa@sheffield.ac.uk)
Mon, 25 Oct 1999 16:25:23 +0100

Hello ABRFers! ,

I wonder if any of you could give me some advice regarding
Acrylamide/polyacrylamide. We are currently using Long Ranger 50 %
solution to make our mixes. Now, the problem we have is that we don't
have enough fumehood space to allow us to pour our gels in them
resulting in us having to pour them on our own individual benches. I
am not happy with this situation and think that there are safety rules
that we are breaking.

Could you tell me

a) How you pour your gels (bench or fumehood)?

b) How it should be done?

c) Is the gel safe after polymerisation (what about the liquid that
settles on the surface of the gel? or dry polyacrylamide dust?). Some
people say this is safe. I can't find any reference to this except
hearsay.

Any help would be appreciated.

Thanks in advance.

Andy Krupa.

Andy Krupa
Dept. of Animal and Plant Sciences
University of Sheffield
Western Bank
Sheffield
S10 2TN

Tel.01142 220108