The "T" blobs you see around 100-150 are due to ethanol precipitation. I
assume you are using ethanol precipitation to clean your sequencing
reactions. These blobs appear because of inadequate removal of 95-100%
ethanol. If you are removing 95% ethanol by decanting, try to remove it by
pippette or aspirate it out.
We had the same problem, now we remove 95% ethanol by carefully pipetting
it out, before washing the pellet with 70% ethanol.
Good Luck.
Saeed Raees
Ana-Gen Technologies, Inc.
4015 Fabian Way
Palo Alto, CA 94303
650-494-3894 Tel
650-494-3893 Fax
saeed@ana-gen.com
http://www.ana-gen.com
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* Mutation Detection
* Gene Synthesis
* Site Directed Mutagenesis
* Oligosynthesis
* Plasmid Purification
* DNA Sequencing
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