I wonder if any of you could give me some advices regarding the solubility
of oligonucleotides after HPLC purification. It happens sometimes and even
for short oligos that a precipate appears after solubilization of dried
oligos in water. All of these oligos have been purified by HPLC. From my
point of view it cannot be any remains of silica. I wonder if it is related
to the water (we use MilliQ water) or to the sequence of the oligos. Most of
the time if we use ý 2% NH3 solution to solubilize the oligos then it works
fine and no precipitate appears.
Any scientific suggestions ?????
Thank you very much
Alain
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Alain LAURENT Ph. D
Oligonucleotide Chemistry
ESGS Groupe CYBERGENE
11, rue Claude Bernard
35 400 Saint MAL0
FRANCE
Tel: +33 (0)2 99 21 90 40
Fax: +33 (0)2 99 21 90 41
e-mail: a.laurent@eurosequence.com
http://www.eurosequence.com
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