you need other buffers, a special loading protocol, and run
conditions in addtion to the increase in data collection time.
With regard to the Seq. Analysis question: According to our knowledge
this is a bug in the Seq. Analysis software. It seems to show up when
you change more than one parameter at once. Under these conditions,
the value for the startpoint and/or the spacing is "jumping" back to
the number prior to change. But if only one parameter is modified,
things should be OK.
I hope I did understand your question correctly.
All the best, Bernd
>Dear Dr, Weisshaar,
>
>Concerning the 1000bp protocol
>
>The 1000bp protocol seems easy enough to use when it comes to turning
>the gel run on and off. But what about the Seq.Anal. How does one extend
>the starting point to 0 (zero) or whatever you want? We have been trying
>to set the Peak 1 Location and start point to zero but the analysis
>program always changes both to at least 300!!!
>I talked to support and they say that it is default for the program to
>look for the Peak 1 location in a window between 800/1100 scans and if
>it does not find it there it sets it to 300.
>
>Have You solved this??
>
>Could You help me with this?
>
>Sincerely
>
>Dr. Jan Karlsson
>Dept. of Microbiology
>NBC-Defence
>Umea
>Sweden
_____________________________________________________________________
PD Dr. Bernd Weisshaar
MPI fuer Zuechtungsforschung
Carl-von-LinnÈ-Weg 10
50829 Koeln
Germany
Tel. office: +49-221-5062 590 Tel. lab: +49-221-5062 311
Fax: +49-221-5062 313
mailto:weisshaa@mpiz-koeln.mpg.de
WWW-URL: http://www.mpiz-koeln.mpg.de/~weisshaa/BW-info.html