Re: DNA syn

Anthony Yeung (AT_Yeung@FCCC.edu)
Wed, 27 Oct 1999 08:43:35 -0400

Dear Alain:
A long time ago, I found that I cannot always fully solubilize 1 micromole
of oligo in 300 microliter of TE buffer. It was kind of sequence dependent.
If I take the pellet and dissolve it in more buffer, I get back a fraction
of the oligos. Life may be worst in water. If your oligo is concentrated,
say 50 to 100 mM in PO4 concentration from the DNA, the water becomes quite
acidic and may further lower the solubility. You can even look forward to
some depurination happening. I hope this experience is relevant to your case.

Tony

At 11:08 AM 10/27/99 +0200, Alain LAURENT wrote:
>Hello ABRF's users
>
>I wonder if any of you could give me some advices regarding the solubility
>of oligonucleotides after HPLC purification. It happens sometimes and even
>for short oligos that a precipate appears after solubilization of dried
>oligos in water. All of these oligos have been purified by HPLC. From my
>point of view it cannot be any remains of silica. I wonder if it is related
>to the water (we use MilliQ water) or to the sequence of the oligos. Most of
>the time if we use ý 2% NH3 solution to solubilize the oligos then it works
>fine and no precipitate appears.
>
>Any scientific suggestions ?????
>
>Thank you very much
>
>
>Alain
>
>
>**************************************
>Alain LAURENT Ph. D
>Oligonucleotide Chemistry
>ESGS Groupe CYBERGENE
>11, rue Claude Bernard
>35 400 Saint MAL0
>FRANCE
>Tel: +33 (0)2 99 21 90 40
>Fax: +33 (0)2 99 21 90 41
>e-mail: a.laurent@eurosequence.com
>http://www.eurosequence.com
>
>**************************************
>
>

************************************
Anthony T. Yeung, Ph.D.
Director, Fannie E. Rippel Biotechnology Facility
Member, Institute for Cancer Research
Fox Chase Cancer Center
7701 Burholme Ave., Philadelphia, PA 19111
Voice: 215-728-2488 FAX: 215-728-3647 email: AT_Yeung@FCCC.edu
Public Key: 4F3A 98D3 AC8B B56D A49B 4CD6 3057 376A 2951 68A4
http://www.fccc.edu/research/labs/yeung/
************************************