Facing the synthesis of the relatively long (>70 AA) peptide I have decided
to do it by a mixed approach, comprising of the stepwise addition of AA and
a few fully protected peptide fragments of 10-11AA each. Would anybody
experienced in peptide synthesis by fully protected peptide fragment
condensation methodology share with me/us some insights about analysis and
purification of these fully protected (Fmoc-ON) fragments by HPLC. Details
about types of columns (packings)used, solvents, gradients, detection, etc.
would be greatly appreciated.
JL
Dr. Jan Lukszo
Peptide Analysis and Synthesis Unit
NIH/NIAID/OSD/SBS
Twinbrook II
12441 Parklawn Drive
Rockville, MD 20852
phone:(301)496-3786
fax:(301)480-2618
E-Mail: lukszo@ncisun1.ncifcrf.gov