AnaSpec (800-452-5530) sells preweighed HBTU/HOBT package. You only need to
add DMF to the bottle. Hope this help.
Bob Lee
Director of the PRL at UIC, RRC
At 08:17 AM 11/4/99 -0500, Singleton, David H wrote:
>Lisa,
>I saw the group reply of alternate suppliers; that is OK, but you'll still
>need to weigh out the reagents into clean bottles. That's a pain. I
>remember from an APS meeting (Nashville?) a vendor sells preweighed kits for
>these synthesizers; I don't remember who.
>
>I've used TBTU on the 430 with less than stellar results. The metering loop
>often clogged, most likely from precipitation. My experience is that
>activators (uroniums) are on the edge of solubility and often clog /
>crystallize in limited volume systmes like peptide synthesizers.
>Substituting one for another is a risky proposition and one must be willing
>to pay the consequences of that endeavour.
>
>I've never tried PyBOP, as that is both expensive and unstable over long
>periods of time. I did use something called ToPipu. It's a more stable
>variety of TBTU. It worked very nicely and gave a stable solution with HOBt
>for 6 weeks. I don't see it in the 1999 Novabiochem catalog anymore and
>can't provide a supplier.
>
>Hope this is helpful.
>
>David H. Singleton
>Scientist
>Pfizer Central Research
>PO Box 8118-101
>Eastern Point Road
>Groton, CT 06340
>-----Original Message-----
>From: lisa bibbs [mailto:bibbs@scripps.edu]
>Sent: Wednesday, November 03, 1999 1:47 PM
>To: Recipients of ABRF List
>Subject: PepSyn: Activator Backorder/?PyBop substitution
>
>
>ABI is backordered on activator. Has anyone tried PyBop on the 430A or 433?
>
>Thanks
>Lisa
>Lisa Bibbs, Ph.D.
>10550 N. Torrey Pines Rd.
>SP3
>La Jolla, CA 92039
>
>http://corefac.scripps.edu
>
>