I've used TBTU on the 430 with less than stellar results. The metering loop
often clogged, most likely from precipitation. My experience is that
activators (uroniums) are on the edge of solubility and often clog /
crystallize in limited volume systmes like peptide synthesizers.
Substituting one for another is a risky proposition and one must be willing
to pay the consequences of that endeavour.
I've never tried PyBOP, as that is both expensive and unstable over long
periods of time. I did use something called ToPipu. It's a more stable
variety of TBTU. It worked very nicely and gave a stable solution with HOBt
for 6 weeks. I don't see it in the 1999 Novabiochem catalog anymore and
can't provide a supplier.
Hope this is helpful.
David H. Singleton
Scientist
Pfizer Central Research
PO Box 8118-101
Eastern Point Road
Groton, CT 06340
-----Original Message-----
From: lisa bibbs [mailto:bibbs@scripps.edu]
Sent: Wednesday, November 03, 1999 1:47 PM
To: Recipients of ABRF List
Subject: PepSyn: Activator Backorder/?PyBop substitution
ABI is backordered on activator. Has anyone tried PyBop on the 430A or 433?
Thanks
Lisa
Lisa Bibbs, Ph.D.
10550 N. Torrey Pines Rd.
SP3
La Jolla, CA 92039