Re: Protein:Preparation of Histones???? help

Hanne Holm Rasmussen (hhr@biokemi.au.dk)
Tue, 09 Nov 1999 17:57:22 +0100

I guess the problem can be traced to the gels. Most likely she runs
acidic gels - or maybe slighty basic. Histones are extremely basic and
will need NEPGHE gels that are perfect, particularly in the pH=11 region.

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lisa bibbs wrote:

> I have a colleague who is follwoing a Methods in Enzymology, vol 170,
> 1989 article by Lennox and Cohen on the "Analysis of Histone Subtypes
> and Their Modified Forms by Polyacrylamide Gel Electrophoresis"
>
> Does anyone have experience with Histone preparation? She is having
> trouble getting bands in her second dimension gel.
>
> Thanks for any help.
>
> Lisa
> Lisa Bibbs, Ph.D.
> 10550 N. Torrey Pines Rd.
> SP3
> La Jolla, CA 92039
>
> http://corefac.scripps.edu