LCQ Calibration
Dan Kirby (dpkirby@mediaone.net)
Wed, 10 Nov 1999 19:55:01 -0500
In response to the question from Thomas D. McClure, to the best of my
knowledge it's still the case that Ultramark must be used to do the
calibration because those specific ions are required by the calibration
program. One way around the problem of Ultramark residue is to do the
calibration by nanospray. This works for all parameters except em gain.
There's something about the s/n of the nanospray signal and how the program
does em calibration that makes this unreliable. So, if you don't have to
calibrate em gain, you can do the whole calibration with a uL or two of the
SOP solution and little or no cleanup is necessary. Use the suggested
infusion method for em gain only using the heated capillary shield that
Finnigan passed out a while ago.
Dan
_______________________
Dan Kirby
Praecis Pharmaceuticals
1 Hampshire Street
Cambridge, MA 02139
dan.kirby@praecis.com
<http://www.praecis.com>