A customer asked me about identifying 3-nitrotyrosine in a protein
through N-terminal sequencing. I got a reference telling how to synthesize
the PTH derivative (Thanks to Mark Crankshaw!) and successfully completed
the synthesis and purification. However, when the moment of truth came and
I loaded the PTH-3-nitrotyrosine onto my Procise I was dissappointed. It
elutes RIGHT ON TOP of DPTU by our HPLC method. I am not aware of anything
I can do about it, but I know that separation chemistry can be different
from lab to lab. Does anyone out there have a system where
PTH-3-nitrotyrosine resolves, or would you like to try? I am willing to
send out the standard I made. I am going to be gone for the next week,
so if anyone is interested, please send an e-mail to Ruba Deeb
(rsdeeb@mail.med.cornell.edu).
Thank you,
Bill
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Bill Enslow
Protein Sequencing Technician
Cornell BioResource Center
Rm 143 Biotechnology Building
phone: 607-254-4848
fax: 607-254-4847
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